Global Mapping of Small RNA-Target Interactions in Bacteria
Sahar Melamed, Asaf Peer, Raya Faigenbaum-Romm, Yair E Gatt, Niv Reiss, Amir Bar, Yael Altuvia, Liron Argaman, Hanah Margalit
Small RNAs (sRNAs) associated with the RNA chaperon protein Hfq are key posttranscriptional regulators of gene expression in bacteria. Deciphering the sRNA-target interactome is an essential step toward understanding the roles of sRNAs in the cellular networks. We developed a broadly applicable methodology termed RIL-seq (RNA interaction by ligation and sequencing), which integrates experimental and computational tools for in vivo transcriptome-wide identification of interactions involving Hfq-associated sRNAs. By applying this methodology to Escherichia coli we discovered an extensive network of interactions involving RNA pairs showing sequence complementarity. We expand the ensemble of targets for known sRNAs, uncover additional Hfq-bound sRNAs encoded in various genomic regions along with their trans encoded targets, and provide insights into binding and possible cycling of RNAs on Hfq. Comparison of the sRNA interactome under various conditions has revealed changes in the sRNA repertoire as well as substantial re-wiring of the network between conditions. Copyright © 2016 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.molcel.2016.07.026Libraries:
| Library name | Growth condition | Number of unique transcripts | Number of interactions |
|---|---|---|---|
| Exponential Phase | LB exponential Phase | 778 | 1004 |
| Stationary Phase | LB Stationary Phase | 1231 | 1776 |
| Iron Limitation | LB + Dipyridyl | 1278 | 1874 |